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KMID : 0880220140520080689
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Journal of Microbiology
2014 Volume.52 No. 8 p.689 ~ p.695
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Bacterial endophyte Sphingomonas sp. LK11 produces gibberellins and IAA and promotes tomato plant growth
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Khan Abdul Latif
Waqas Muhammad
Kang Sang-Mo
Al-Harrasi Ahmed
Hussain Javid
Al-Rawahi Ahmed
Al-Khiziri Salima
Ullah Ihsan
Ali Liaqat
Jung Hee-Young
Lee In-Jung
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Abstract
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Plant growth promoting endophytic bacteria have been identified as potential growth regulators of crops. Endophytic bacterium, Sphingomonas sp. LK11, was isolated from the leaves of Tephrosia apollinea. The pure culture of Sphingomonas sp. LK11 was subjected to advance chromatographic and spectroscopic techniques to extract and isolate gibberellins (GAs). Deuterated standards of [17, 17-2H2]-GA4, [17, 17-2H2]-GA9 and [17, 17-2H2]-GA20 were used to quantify the bacterial GAs. The analysis of the culture broth of Sphingomonas sp. LK11 revealed the existence of physiologically active gibberellins (GA4: 2.97 ± 0.11 ng/ml) and inactive GA9 (0.98 ± 0.15 ng/ml) and GA20 (2.41 ± 0.23). The endophyte also produced indole acetic acid (11.23 ± 0.93 μM/ml). Tomato plants inoculated with endophytic Sphingomonas sp. LK11 showed significantly increased growth attributes (shoot length, chlorophyll contents, shoot, and root dry weights) compared to the control. This indicated that such phyto-hormones-producing strains could help in increasing crop growth.
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KEYWORD
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Solanum lycopersicum, Sphingomonas sp. LK11, endophytism, gibberellins, indole acetic acid
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